server working

everything is ok now

MLPA server

The application continues down. If you are interesting to mount your own server, email to lpantano@imppc.org to get assistance.

Server down

We have our server temporally down. It will be published here when the server works again. Hope in a few days.

Stand alone version

First, this is the first version, so there will be many bugs that I will fix every day. I will do a complete tutorial in two weeks, to teach how to install and use it correctly. Also, I will develop a version for windows. Thanks in advance!

Requirements:

1. Mac OSX
2. Linux
3. The same RAM memory than your genome is (for human, 2.5 GB).
   
4. perl
5. Install Tk library:

perl -MCPAN -e shell
cpan> install Bundle::CPAN
cpan> reload cpan
cpan> install Tk


6. Install Bioperl:http://bioperl.open-bio.org/wiki/Installing_BioPerl

How to use it:

1. Download the zip file
2. Create a directory whatever you want
3. Unzip the file (dbl click)
4. Open a Terminal and go to the folder where is the file "proseek.pl"
   
5. type: perl proseek.pl
6. You will see the same parameters than in the web page
7. Fisrt you have to load the blat server. For that, you need to download all the chromosomes of the interested genome. From human click here. And save the fasta files in some folder that only contain the fasta files.
8. In the ProSeeK windows, click in Choose button. Select one chromosome of your genome.
9. Click in Load Blat button
10. Wait until in the terminal appears "Server ready for queries!"
    
11. Then, you can paste your sequence in the corresponding text and perform the analysis for any sequence. You have to wait until a new window showing the results appears to perform the next design.
12. Before closing ProSeeK please, click in Stop Blat button to release your memory RAM.
    

New version

On 8/11/2009 a new version of ProSeeK will be able to download to run locally in the user's computer to avoid problems with the server.

Thanks all of you

Server DOWN again

Sorry for the inconvenience but the server hosting ProSeeK is not very stable at this moment. We are trying ti get a new server more efficient.

SERVER RESTORED

ProSeeK works again!